Barrie Ingham .... Basil of Baker Street/Bartholomew

Vincent Price .... Professor Ratigan

Val Bettin .... Dr. David Q. Dawson/Thug Guard

Candy Candido .... Fidget the Bat

Alan Young .... Hiram Flaversham

Susanne Pollatschek .... Olivia Flaversham

Diana Chesney .... Mrs. Judson

Eve Brenner .... Queen Mousetoria (aka The Mouse Queen)

Melissa Manchester .... Miss Kitty Mouse

Shani Wallis .... Lady Mouse

Ellen Fitzhugh .... Barmaid

Basil Rathbone (recording from Red-Headed League) .... Sherlock Holmes

Laurie Main .... Dr. John H. Watson

Walker Edmiston .... Citizen/Thug Guard

Wayne Allwine .... Thug Guard

Tony Anselmo .... Thug Guard

Frank Welker (uncredited) .... Toby/Felicia/Royal Guard Dogs